Passa al contenuto

PCR Purification Kit

https://www.neurovasx.com/web/image/product.template/45863/image_1920?unique=0936d89
(0 recensione)

High-Throughput PCR Product Purification Kit – 96-Well Format

This 96-well PCR purification kit offers a rapid and efficient method for high-throughput purification of amplified DNA products. It effectively removes common PCR by-products such as primers, primer dimers, enzymes, unincorporated nucleotides, and residual mineral oil, yielding clean DNA suitable for downstream applications. The purified DNA is fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing, and other molecular biology workflows. This kit also provides a convenient alternative to traditional organic extraction and ethanol precipitation for cleaning up various enzymatic reactions.

Purification Technology

Purification is based on 96-well column chromatography, which allows DNA binding under high-salt conditions and elution under low-salt, slightly alkaline conditions. To begin, PCR products are mixed with a provided binding buffer at a 1:5 ratio. Mineral oil from PCR reactions does not need to be removed, as it is effectively separated during the purification process. The mixture is then applied to the 96-well plate using either centrifugation or a vacuum manifold. DNA binds to the plate based on ionic interactions, while primers, dimers, enzymes, and other contaminants flow through.

Bound DNA is washed twice with the supplied wash solution to remove residual impurities, and highly purified PCR products are finally eluted using the provided elution buffer. This process produces clean, high-quality DNA suitable for all common molecular biology applications, including cloning, sequencing, labeling, and enzymatic reactions.

Advantages

  • Rapid and convenient processing using either a vacuum manifold or centrifugation
  • Effective removal of all PCR by-products, including primers and primer dimers
  • Superior primer removal efficiency, typically exceeding 90%
  • High DNA recovery, with purified PCR products exceeding 90% of the original input

Key Features:

  • High-throughput purification in 96-well format
  • Rapid removal of primers, dimers, enzymes, and nucleotides
  • No need to remove mineral oil from PCR reactions
  • Compatible with restriction digestion, ligation, labeling, and sequencing
  • Alternative to organic extraction and ethanol precipitation
  • Simple workflow using vacuum or centrifugation

Applications:

  • PCR product cleanup
  • Enzymatic reaction purification
  • DNA sequencing preparation
  • Cloning and ligation workflows
  • High-throughput molecular biology projects
PCR Purification Kit

485,00 485.0 USD 485,00

485,00

Not Available For Sale

Questa combinazione non esiste.

pcr

Termini e condizioni
Garanzia di rimborso di 30 giorni
Spedizione: 2-3 giorni lavorativi