Fluorescent Dopaminergic Receptor D3 Internalization Assay Cell Line

Description Green Fluorescent D3 Dopamine Receptor Cell Line contains SH-SY5Y cells stably expressing DRD3 Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual. The Green Fluorescent D3 Dopamine Receptor Cell Line allows to assay compounds, or analyze their capability to modulate DRD3 Receptor activation and the following redistribution process inside the cells. When a ligand binds to the DRD3 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent D3 Dopamine Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring DRD3 Receptor redistribution process in High Throughput Screening.