Fluorescent human chemokine (C-X-C motif) receptor 2 Internalization Assay Cell Line

Description Green Fluorescent CXCR2 Chemokine Receptor Cell Line contains U2OS cells stably expressing CXCR2 tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual. The Green Fluorescent CXCR2 Chemokine Receptor Cell Line allows to assay compounds, or analyze their capability to modulate CXCR2 activation and the following redistribution process inside the cells. When a ligand binds to the CXCR2 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent CXCR2 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring CXCR2 Receptor redistribution process in High Throughput Screening.