Fluorescent Human Formyl Peptide Receptor 2 Internalization Assay Cell Line

Description Green Fluorescent FPR2/ALX Formylpeptide Receptor Cell Line contains U2OS cells stably expressing FPR2/ALX tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual. The Green Fluorescent FPR2/ALX Formylpeptide Receptor Cell Line allows to assay compounds, or analyze their capability to modulate FPR2/ALX activation and the following redistribution process inside the cells. When a ligand binds to the FPR2/ALX Formylpeptide Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent FPR2/ALX redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring FPR2/ALX Formylpeptide Receptor redistribution process in High Throughput Screening.